| 475 | 0 | 74 |
| 下载次数 | 被引频次 | 阅读次数 |
[目的]大量研究表明，LEA蛋白能够通过多种机制帮助植物应对环境压力，在水稻中仍有大量LEA家族成员的功能尚未得到充分验证。因此，通过对水稻LEA基因家族(OsLEA)的生物信息学分析，并结合其在不同胁迫下的基因表达变化，旨在挖掘参与水稻中响应非生物胁迫的OsLEA新成员。[方法]采用全基因组生物信息学方法对OsLEA基因家族进行了鉴定和分析。[结果]水稻基因组中共包含83个OsLEA基因家族成员，这些基因不均匀地分布在12条染色体上，根据其结构域特征可分为8个亚家族。理化性质分析显示，LEA蛋白具有广泛的亲水性和较强的稳定性。亚细胞定位预测表明，大多数LEA蛋白分布在细胞质、细胞膜和线粒体等细胞组分中。启动子顺式作用元件分析表明，OsLEA基因的启动子区域富含与非生物胁迫、植物激素响应和生长发育相关的元件。组织表达模式和激素响应分析揭示，OsLEA基因在不同组织和不同植物激素处理中表现出显著的表达特异性。此外，非生物胁迫（低温、高温、盐和重金属镉）处理下，部分OsLEA基因表现出明显的应答。[结论]通过整合生物信息学分析及多种激素与逆境胁迫下的表达谱数据，初步揭示了OsLEA基因家族参与逆境响应的分子调控机制。基于此，成功筛选出Os04g0589800、Os05g0362600、Os06g0324400和Os08g0163600等核心成员基因，其在高盐、高温及低温胁迫下均呈现显著表达上调特征，证实了它们在非生物胁迫应答中的关键生物学功能，为抗逆水稻育种提供了极具应用潜力的分子靶标。
Abstract：[Objective]A large number of studies have shown that LEA proteins can help plants cope with environmental stress through various mechanisms. However, the functions of many LEA family members in rice have not been fully verified. Therefore, this study aimed to identify new LEA members involved in rice responses to abiotic stress through bioinformatics analysis of the OsLEA gene family and by combining their gene expression changes under different stresses. [Method]The OsLEA gene family was identified and analysed using a genome-wide bioinformatics approach. [Results]The results reveal that the rice genome contains 83 OsLEA family members, which are unevenly distributed across 12 chromosomes and can be classified into eight subfamilies based on structural domain features. Physicochemical property analysis shows that LEA proteins are highly hydrophilic and stable. Subcellular localization predictions indicate that most LEA proteins are localized in cellular compartments such as the cytoplasm, cell membrane,and mitochondria. Promoter cis-acting element analysis highlights that the OsLEA gene promoters are enriched with elements associated with abiotic stress response, phytohormone regulation, and growth and development. Tissue expression pattern and hormone response analyses reveal significant expression specificity across different tissues and under various phytohormone treatments.Additionally, certain OsLEA genes exhibit notable responses to abiotic stresses such as low and high temperatures, salt, and heavy metal(cadmium) treatments. These findings provide valuable insights into the role of OsLEA genes in coping with environmental stress.[Conclusion]This study integrated bioinformatics analysis with expression profiling data under multiple hormone treatments and abiotic stress conditions, preliminarily revealing the molecular regulatory mechanisms through which the OsLEA gene family participates in stress responses. Building on these findings, we successfully screened core member genes—including Os04g0589800,Os05g0362600, Os06g0324400, and Os08g0163600—which exhibited significant upregulation under high-salinity, high-temperature,and low-temperature stresses. This confirms their critical biological functions in abiotic stress responses and establishes them as highly promising molecular targets for breeding stress-resistant rice cultivars.
[1] ARTUR M A S,ZHAO T,LIGTERINK W,et al.Dissecting the genomic diversification of late embryogenesis abundant(LEA)protein gene families in plants[J].Genome Biology and Evolution,2019,11(2):459-471.
[2] MERTENS J,ALIYU H,COWAN D A.LEA proteins and the evolution of the WHy domain[J].Applied and Environmental Microbiology,2018,84(15):e00539-18.
[3] DOS REIS S P,LIMA A M,DE SOUZA C R B.Recent molecular advances on downstream plant responses to abiotic stress[J].International Journal of Molecular Sciences,2012,13(7):8628-8647.
[4]李乐,许红亮,杨兴露,等.大豆LEA基因家族全基因组鉴定、分类和表达[J].中国农业科学,2011,44(19):3945-3954.LI L,XU H L,YANG X L,et al.Genome-wide identification,classification and expression analysis of LEA gene family in Soybea[J].Scientia Agricultura Sinica,2011,44(19):3945-3954.
[5]陈凯,佟晓楠,张晓媛,等.枳LEA基因家族鉴定及其对非生物胁迫的响应[J].西北植物学报,2023,43(6):918-928.CHEN K,TONG X N,ZHANG X Y,et al.Genome-wide identification and abiotic stress responses of LEA gene family in Poncirus trifoliata[J].Acta Botanica Boreali-Occidentalia Sinica,2023,43(6):918-928.
[6] SUN Z P,LI S Y,CHEN W Y,et al.Plant dehydrins:expression,regulatory networks,and protective roles in plants challenged by abiotic stress[J].International Journal of Molecular Sciences,2021,22(23):12619.
[7] CANDAT A,PASZKIEWICZ G,NEVEU M,et al.The ubiquitous distribution of late embryogenesis abundant proteins across cell compartments in Arabidopsis offers tailored protection against abiotic stress[J].The Plant Cell,2014,26(7):3148-3166.
[8] WU C L,HU W,YAN Y,et al.The late embryogenesis abundant protein family in cassava(Manihot esculenta Crantz):Genomewide characterization and expression during abiotic stress[J].Molecules,2018,23(5):1196.
[9] LIANG Y,XIONG Z Y,ZHENG J X,et al.Genome-wide identification,structural analysis and new insights into late embryogenesis abundant(LEA)gene family formation pattern in Brassica napus[J].Scientific Reports,2016,6:24265.
[10] SHIH M D,HUANG L T,WEI F J,et al.OsLEA1a,a new Em-like protein of cereal plants[J].Plant&Cell Physiology,2010,51(12):2132-2144.
[11] XIAO B Z,HUANG Y M,TANG N,et al.Over-expression of a LEA gene in rice improves drought resistance under the field conditions[J].Theoretical and Applied Genetics,2007,115(1):35-46.
[12] DUAN J L,CAI W M.OsLEA3-2,an abiotic stress induced gene of rice plays a key role in salt and drought tolerance[J].PLoS One,2012,7(9):e45117.
[13] HU T Z,LIU Y L,ZHU S S,et al.Overexpression of OsLea14-a improves the tolerance of rice and increases Hg accumulation under diverse stresses[J].Environmental Science and Pollution Research International,2019,26(11):10537-10551.
[14] YU J,LAI Y M,WU X,et al.Overexpression of OsEm1 encoding a group I LEA protein confers enhanced drought tolerance in rice[J].Biochemical and Biophysical Research Communications,2016,478(2):703-709.
[15] GANGULY M,DATTA K,ROYCHOUDHURY A,et al.Overexpression of Rab16A gene in indica rice variety for generating enhanced salt tolerance[J].Plant Signaling&Behavior,2012,7(4):502-509.
[16] HU T Z,ZENG H,HE S,et al.Molecular analysis of OsLEA4 and its contributions to improve E.coli viability[J].Applied Biochemistry and Biotechnology,2012,166(1):222-233.
[17] HUANG L P,ZHANG M Y,JIA J,et al.An atypical late embryogenesis abundant protein OsLEA5 plays a positive role in ABA-induced antioxidant defense in Oryza sativa L[J].Plant&Cell Physiology,2018,59(5):916-929.
[18]薛亚莉,魏晓双,周海连,等.水稻LEA2家族的鉴定与表达谱分析[J].华中农业大学学报,2021,40(2):93-102.XUE Y L,WEI X S,ZHOU H L,et al.Characterization and expression profile analysis of LEA_2 family in rice[J].Journal of Huazhong Agricultural University,2021,40(2):93-102.
[19]张琪,刘爽,胡艳娟,等.水稻OsRIP基因家族的全基因组鉴定及其表达分析[J].沈阳农业大学学报,2023,54(4):385-395.ZHANG Q,LIU S,HU Y J,et al.Genome-wide identification and expression analysis of the OsRIP gene family in rice[J].Journal of Shenyang Agricultural University,2023,54(4):385-395.
[20]陈宏伟,商文奇,吕桂兰,等.东北粳稻穗部性状全基因组关联分析[J].沈阳农业大学学报,2025,56(3):23-32.CHEN H W,SHANG W Q,LüG L,et al.Genome-wide association study of panicle traits in japonica rice from Northeast China[J].Journal of Shenyang Agricultural University,2025,56(3):23-32.
[21] BIES-ETHèVE N,GAUBIER-COMELLA P,DEBURES A,et al.Inventory,evolution and expression profiling diversity of the LEA(late embryogenesis abundant)protein gene family in Arabidopsis thaliana[J].Plant Molecular Biology,2008,67(1/2):107-124.
[22] HUNDERTMARK M,HINCHA D K.LEA(late embryogenesis abundant)proteins and their encoding genes in Arabidopsis thaliana[J].BMC Genomics,2008,9:118.
[23] NAGARAJU M,KUMAR S A,REDDY P S,et al.Genome-scale identification,classification,and tissue specific expression analysis of late embryogenesis abundant(LEA)genes under abiotic stress conditions in Sorghum bicolor L[J].PLoS One,2019,14(1):e0209980.
[24] ZHANG Y P,ZHANG X J,ZHU L J,et al.Identification of the maize LEA gene family and its relationship with kernel dehydration[J].Plants,2023,12(21):3674.
[25] ZHAO P S,LIU F,MA M,et al.Overexpression of AtLEA3-3 confers resistance to cold stress in Escherichia coli and provides enhanced osmotic stress tolerance and ABA sensitivity in Arabidopsis thaliana[J].Molekuliarnaia Biologiia,2011,45(5):851-862.
[26] JIA H L,WANG X M,SHI Y H,et al.Overexpression of Medicago sativa LEA4-4 can improve the salt,drought,and oxidation resistance of transgenic Arabidopsis[J].PLoS One,2020,15(6):e0234085.
[27] NAKASHIMA K,ITO Y,YAMAGUCHI-SHINOZAKI K.Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses[J].Plant Physiology,2009,149(1):88-95.
[28] NIU B X,DENG H,LI T T,et al.OsbZIP76 interacts with OsNF-YBs and regulates endosperm cellularization in rice(Oryza sativa)[J].Journal of Integrative Plant Biology,2020,62(12):1983-1996.
[29] WANG J C,XU H,ZHU Y,et al.OsbZIP58, a basic leucine zipper transcription factor,regulates starch biosynthesis in rice endosperm[J].Journal of Experimental Botany,2013,64(11):3453-3466.
[30] KIM J A,BHATNAGAR N,KWON S J,et al.Transcriptome analysis of ABA/JA-dual responsive genes in rice shoot and root[J].Current Genomics,2018,19(1):4-11.
[31] VERMA V,RAVINDRAN P,KUMAR P P.Plant hormone-mediated regulation of stress responses[J].BMC Plant Biology,2016,16:86.
[32] SONG C,CAO Y P,DAI J,et al.The multifaceted roles of MYC2in plants:Toward transcriptional reprogramming and stress tolerance by jasmonate signaling[J].Frontiers in Plant Science,2022,13:868874.
[33] NAKASHIMA K,YAMAGUCHI-SHINOZAKI K.ABA signaling in stress-response and seed development[J].Plant Cell Reports,2013,32(7):959-970.
基本信息:
DOI：
中图分类号:S511
引用信息:
[1]赵贺,冀亚亮,梁潇,等.水稻LEA基因家族的全基因组鉴定及综合分析[J].沈阳农业大学学报,2025,56(05):14-25.
基金信息:
辽宁省科技重大专项项目(2024JH1/11700006)