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[目的]植物在缺氮条件下可通过促进初生根生长增强对氮源的吸收，但其分子机制尚未明晰。旨在揭示水稻响应氮缺乏时根系形态适应的内在机理，以阐明初生根生长调控与氮素吸收效率之间的关联。[方法]以水稻野生型与Osdgl1突变体为试验材料，设置正常供氮与缺氮处理,观察并测定初生根生长表型，同时分析根系细胞壁主要成分（包括鼠李糖、木糖和纤维素）的含量变化。[结果]缺氮处理显著促进水稻初生根伸长，并部分恢复Osdgl1突变体的短根表型。遗传证据表明OsDGL1在缺氮诱导的根系生长过程中发挥关键作用。进一步分析发现，根系长度变化主要与细胞壁中鼠李糖与木糖的含量相关，而与纤维素无关，表明鼠李糖生物合成可能在该过程中起重要作用。[结论]OsDGL1可能通过调控鼠李糖的生物合成途径，介导缺氮条件下水稻初生根的生长响应。为解析氮素缺乏调控水稻根系发育的分子机制提供了新的线索与研究方向。
Abstract：[Objective]Under nitrogen-deficient conditions, plants can enhance nitrogen uptake by promoting primary root growth; yet the molecular mechanisms underlying this adaptive response remain poorly understood. This study aimed to elucidate the intrinsic regulatory mechanisms of root morphological adaptation in rice in response to nitrogen deficiency, with a specific focus on clarifying the relationship between primary root growth regulation and nitrogen uptake efficiency. [Methods]Wild-type rice and the Osdgl1 mutant were used as experimental materials. Plants were subjected to either normal nitrogen supply or nitrogen-deficient conditions.Primary root growth phenotypes were systematically observed and measured. Concurrently, changes in the content of root cell wall components—including rhamnose, xylose, and cellulose, were analyzed. [Results]Nitrogen-deficient treatment significantly promoted primary root elongation of rice and partially restored the short-root phenotype of the Osdgl1 mutant. Genetic evidence indicated that OsDGL1 plays a crucial role in nitrogen deficiency-induced root growth. Further analysis revealed that changes in root length were primarily correlated with the content of rhamnose and xylose in the cell wall, but not with cellulose content. These findings suggest that rhamnose biosynthesis may serve an important function in this adaptive process. [Conclusion]OsDGL1 likely mediates the growth response of rice primary roots under nitrogen-deficient conditions by regulating the rhamnose biosynthesis pathway. This study provides novel insights and identifies future research directions for unraveling the molecular mechanisms through which nitrogen deficiency modulates root development in rice.
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基本信息:
中图分类号:S511
引用信息:
[1]袁陈,张燕莉,赵明辉,等.OsDGL1介导的细胞壁鼠李糖代谢参与调控水稻对缺氮诱导的初生根伸长响应[J].沈阳农业大学学报,2026,57(03):10-16.
基金信息:
辽宁省科技重大专项项目（2024JH1/11700006-3）; 国家重点研发计划项目（2024YFD1501505）
2026-04-16
2026-04-16
2026-04-16