| 152 | 0 | 21 |
| 下载次数 | 被引频次 | 阅读次数 |
[目的]猫瘟是由猫细小病毒引起的猫的传染性疾病，其主要特点是白细胞数量下降和出血性胃肠炎，具有较高的感染率和致死率，对宠物的健康造成严重威胁。疫苗接种是目前预防猫瘟最主要的方式，但疫苗的免疫效果受到多种因素的制约。免疫增强剂能够增强畜禽的疫苗免疫效果，探究免疫增强剂对宠物疫苗免疫效果的影响，以期为猫的免疫防治效果提供理论依据。[方法]选择幼猫作为试验对象，基于单因子试验设计方案，各试验组分别在60,81,102日龄免疫妙三多疫苗，免疫前后4 d分别饲喂0.9%氯化钠生理盐水（对照组）、黄芪多糖（APC组）、脾氨肽（SAT组）和高免因子核酸制剂（NAP组），对各组幼猫IgG抗体、IFN-γ及全血细胞分类进行检测。[结果]脾氨肽和高免因子核酸制剂能够提升幼猫猫瘟抗体和INF-γ的分泌水平以及白细胞和中性粒细胞的数量，但黄芪多糖对幼猫猫瘟抗体和INF-γ的分泌水平等无显著效果；脾氨肽和高免因子核酸制剂对幼猫的红细胞、血小板、单核细胞和淋巴细胞数量也无显著提升。[结论]脾氨肽和高免因子核酸制剂能够显著提高幼猫体内部分抗体指标水平，表明这两类试剂可以提高幼猫对猫瘟的免疫效果。该研究为幼猫免疫增强剂的研究奠定初步的理论基础，为进一步研究猫瘟等疾病的有效防治积累数据，同时对于家禽和家畜常见免疫增强试剂用于增强幼猫等宠物的免疫力提供参考。
Abstract：[Objective]Feline distemper is an infectious disease of cats caused by feline microvirus, which is mainly characterised by decreased leukocyte counts and haemorrhagic gastroenteritis, and has a high rate of infection and lethality, posing a serious threat to the health of pets. Vaccination is currently the most important way to prevent feline distemper, but the immune effect of the vaccine is constrained by a variety of factors. Immunity enhancers can enhance the effect of vaccine immunity in livestock and poultry. We explored the effect of immunity enhancers on the effect of vaccine immunity in pets, aiming to provide a theoretical basis for the effect of immunity prevention and control in cats. [Methods]Young cats were selected as experimental subjects. Based on a one-way experimental design, each experimental group was immunised with Myo-Santo vaccine at 60, 81 and 102 days of age, and fed with0.9% NaCl saline(control group), Astragali polysaccharides(APC group), splenic aminotransferase peptide(SAT group), and high immunity factor nucleic acid preparations(NAP group), respectively, for 4 d before and after immunization. All groups were examined for their IgG antibody, IFN-γ and whole blood cell classification were detected. [Results]Splenic aminopeptide and high immunity factor nucleic acid preparation were able to increase the secretion levels of distemper antibody and INF-γ, as well as the number of leukocytes and neutrophils in young cats, but astragali polysaccharides did not have a significant effect on the secretion levels of distemper antibody and INF-γ in young cats; and splenic aminopeptide and high immunity factor nucleic acid preparation did not have a significant effect on the number of erythrocytes, platelets, monocytes, and lymphocytes in young cats. [Conclusion]Splenic aminopeptide and high immunity factor nucleic acid preparations were able to significantly increase the levels of some antibody indexes in young cats, suggesting that these two reagents can improve the immunity effect of young cats against feline distemper. This study lays a preliminary theoretical foundation for the study of immune enhancers in young cats, accumulates data for further research on the effective prevention and treatment of feline distemper and other diseases, and provides a reference for the use of common immune-enhancing reagents for poultry and livestock to enhance the immunity of young cats and other pets.
[1] BARRS V R.Feline panleukopenia:A re-emergent disease[J].The Veterinary Clinics of North America Small Animal Practice,2019,49(4):651-670.
[2] STUETZER B,HARTMANN K.Feline parvovirus infection and associated diseases[J].The Veterinary Journal,2014,201(2):150-155.
[3] GUIMAR?ES L E,BAKER B,PERRICONE C,et al.Vaccines,adjuvants and autoimmunity[J].Pharmacological Research,2015,100:190-209.
[4]张齐,刘朋,杨诚洁,等.口服表面活性素对母猪乳源性免疫力的影响[J].南京农业大学学报,2024,47(6):1122-1129.ZHANG Q,LIU P,YANG C J,et al.Effects of oral surface active agents on lactogenic immunity of sows[J].Journal of Nanjing Agricultural University,2024,47(6):1122-1129.
[5]邵长轩,赵光辉,朱永杰,等.人源Cathelicidins家族抗菌肽LL-37生物学功能和分子修饰研发进展[J].东北农业大学学报,2023,54(7):80-90.SHAO C X,ZHAO G H,ZHU Y J,et al.Biological function and molecular modification of LL-37,a human-derived antimicrobial peptide of Cathelicidins family[J].Journal of Northeast Agricultural University,2023,54(7):80-90.
[6]杜露平,侯立婷,于晓明,等.免疫增强剂CVC1302辅助抗原诱导小鼠机体产生长效体液免疫应答的研究[J].南京农业大学学报,2022,45(3):597-603.DU L P,HOU L T,YU X M,et al.Study on the generation of long-lasting humoral immune response in mouse organism induced by immune enhancer CVC1302 adjuvant antigen[J].Journal of Nanjing Agricultural University,2022,45(3):597-603.
[7] TANG Z J,HUANG G L.Extraction,structure,and activity of polysaccharide from Radix astragali[J].Biomedicine&Pharmacotherapy,2022,150:113015.
[8]唐文雅,聂福旭,刘根新,等.黄芪多糖和枯草芽孢杆菌合剂对乳鸽生长性能和免疫功能的影响[J].现代畜牧兽医,2024(3):37-41.TANG W Y, NIE F X, LIU G X,et al. Effects of a combination of Astragalus polysaccharide and Bacillus subtilis on growth performance and immune function of pigeons[J].Modern Animal Husbandry and Veterinary Medicine,2024(3):37-41.
[9]邓姣,李紫晗,唐炳,等.黄芪多糖类在猪肠道健康和免疫功能中的应用研究[J].猪业科学,2023,40(12):96-98.DENG J,LI Z H,TANG B,et al.Application of astragalus polysaccharides in intestinal health and immune function of pigs[J].Swine Science,2023,40(12):96-98.
[10]梁秋亭,谢衬梨,王惠嫦,等.胸腺肽α1对铜绿假单胞菌感染支气管扩张症大鼠的免疫调节作用[J].中国医药科学,2023,13(23):21-24,81.LIANG Q T,XIE L L,WANG H X,et al.Immunomodulatory effects of thymopeptide α1 on Pseudomonas aeruginosa-infected rats with bronchiectasis[J].Chinese Medical Science,2023,13(23):21-24,81.
[11]柳丛.脾氨肽口服冻干粉治疗免疫功能紊乱相关疾病的机制与效果研究进展[J].临床合理用药,2023,16(25):167-170.LIU C.Mechanism and effect of oral lyophilized powder of splenic aminopeptide in the treatment of immune dysfunction-related diseases[J].Clinical Rational Use of Drugs,2023,16(25):167-170.
[12]彭苏予,吴秋红,包松松,等.几种免疫增强剂在水产养殖中的应用研究进展[J].科学养鱼,2023(7):74-76.PENG S Y,WU Q H,BAO S S,et al.Progress of application of several immune enhancers in aquaculture[J].Scientific Fish Culture,2023(7):74-76.
[13]徐端红,贾新彦,胡梅.黄芪多糖对断奶羔羊生长性能及免疫力的影响[J].中国饲料,2018(12):56-59.XU D H,JIA X Y,HU M.Effects of astragalus polysaccharide on growth performance and immunity of weaned lambs[J].China Feed,2018(12):56-59.
[14]曾涵芳,李烨青,王泽栋,等.黄芪多糖对热应激下奶牛生产性能、血清生化指标的影响[J].中国奶牛,2019(12):42-49.ZENG H F,LI Y Q,WANG Z D,et al.Effects of astragalus polysaccharide on performance and serum biochemical indexes of dairy cows under heat stress[J].China Dairy Cattle,2019(12):42-49.
[15]柏雪,陈燕燕,马婉婷,等.天然植物多糖的动物免疫调节功能研究进展[J].西南民族大学学报(自然科学版),2024,50(4):367-375.BER X,CHEN Y Y,MA W T,et al.Progress of immunomodulatory functions of natural plant polysaccharides in animals[J].Journal of Southwest Minzu University(Natural Science Edition),2024,50(4):367-375.
[16]刘磊.黄芪多糖调控肉鸡免疫应激反应研究[D].杨凌:西北农林科技大学,2015.LIU L.Astragalus polysaccharide regulates the immune stress response in broiler chickens[D].Yangling:Northwest Agriculture and Forestry University,2015.
[17]叶建敏,管敏昌,汪官富,等.儿童难治性支原体肺炎血液T淋巴细胞亚群水平及脾氨肽冻干粉对其影响[J].中国卫生检验杂志,2019,29(3):294-296.YE J M,GUAN M C,WANG G F,et al.Blood T-lymphocyte subpopulation levels of refractory mycoplasma pneumonia in children and the effect of splenic aminotransferrin lyophilized powder on them[J].Chinese Journal of Health Inspection,2019,29(3):294-296.
[18]田欣逸,王军,王洪青,等.脾氨肽对巨细胞病毒感染儿童CD4+、CD8+及免疫球蛋白的影响[J].热带医学杂志,2020,20(9):1218-1221.TIAN X Y,WANG J,WANG H Q,et al.Effect of splenic aminopeptide on CD4+,CD8+and immunoglobulins in cytomegalovirus-infected children[J].Journal of Tropical Medicine,2020,20(9):1218-1221.
[19]李彧,田颖,杨晓梅,等.胸腺肽α1对慢性乙型肝炎CD4+CD25+CD127dim/-调节性T淋巴细胞功能的影响[J].临床肝胆病杂志,2018,34(5):1011-1014.LI Y,TIAN Y,YANG X M,et al.Effect of thymosin α1 on CD4+CD25+CD127dim/-regulatory T lymphocyte function in chronic hepatitis B[J].Journal of Clinical Hepatobiliary Diseases,2018,34(5):1011-1014.
[20]陈闯嘉宝,黄秉洲,邓李霜,等.胸腺肽的免疫调节活性及其在病毒性疾病中的应用[J].畜牧与兽医,2023,55(11):138-144.CHEN G J B, HUANG B Z,DANG L S,et al.Immunomodulatory activity of thymic peptides and its application in viral diseases[J].Livestock and Veterinary Medicine,2023,55(11):138-144.
[21]白洁,徐斌,黄彦鸣,等.黄芪多糖对史宾格犬疫苗的免疫效果[J].中南农业科技,2024(6):44-47.BAI J,XU B,HUANG Y M,et al.Immunization effect of Astragalus polysaccharide on Schizosaccharomyces pallidus vaccine[J].Zhongnan Agricultural Science and Technology,2024,45(6):44-47.
[22]彭艳辉,邓莉莉,周云,等.脾氨肽对急性淋巴细胞白血病患儿维持治疗期间免疫功能的影响[J].海南医学,2019,30(14):1776-1779.PENG Y H,DANG L L,ZHOU Y,et al.Effect of splenic aminopeptide on immune function during maintenance therapy in children with acute lymphoblastic leukemia[J].Hainan Medical Science,2019,30(14):1776-1779.
基本信息:
DOI：
中图分类号:S858.293
引用信息:
[1]于立辉,孙小云,王浩然等.不同免疫增强剂对猫瘟疫苗免疫效力的影响[J].沈阳农业大学学报,2025,56(02):63-68.
基金信息:
沈阳农业大学2023年大学生创新创业训练计划项目（D202305230810479591,D202305161507454709）; 沈阳农业大学兽医产科学线下一流课建设项目（2022）